CD19 CoraLite Plus 405 (4G7, Mouse) - Reactivity: Human

About CD19
This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]

About CoraLite Plus 405
CoraLite Plus 405 from Proteintech has an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to DyLight™ 405 and Alexa Fluor™ 405 and can be useful in fluorescence microscopy and flow cytometry. CoraLite® Plus dyes are chemically engineered with SuperHydrophilic dPEG® groups for greater biocompatibility, high water solubility, enhanced brightness and photostability. The family of CoraLite® Plus fluorophores are spectrally similar to Alexa Fluor® dyes.