IgM / CF770 /

Product Details
Supplier Biotium
Catalog #: #20385-250uL (View supplier product page)
Size 250 μl
Price $215.00
Antigen IgM
Clone
Host Goat
Isotype IgG
Conjugate CF770
Target Species Mouse
Applications IF, IHC, WB
Description Goat anti-mouse IgM (μ chain) labeled with CF®770 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent CF®770 dye has excitation/emission at 770/797 nm. The conjugate is prepared from antibodies purified by mouse IgM-affinity chromatography. The antibodies react specifically with IgM heavy chains (μ chains) and not with immunoglobulin light chains.
About IgM and CF770
IgM Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
CF770 CF®770 from Biotium is a fluorophore with an excitation peak at 770 nm and an emission peak at 797 nm. It is spectrally similar to DyeLight 800 and IRDye® 800CW.
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CF770 Excitation and Emission Spectra