IgG / CF405S /

Product Details
Supplier Biotium
Catalog #: #20420-500uL (View supplier product page)
Size 500 μl
Price $252.00
Antigen IgG
Clone
Host Donkey
Isotype IgG
Conjugate CF405S
Target Species Rabbit
Applications IF, IHC, WB
Description This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF®405S dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®405S dye has excitation/emission at 404/431 nm. Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.
About IgG and CF405S
IgG This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
CF405S CF®405S from Biotium is a fluorophore with an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to Alexa Fluor™ 405. It can be use in fluorescence microscopy, specifically its been validated in Structured Illumination (SIM), and flow cytometry. However, in flow cytometry, superior alternatives are Brilliant™ Violet 421, SuperNova v428 and StarBright Violet 440.
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CF405S Excitation and Emission Spectra