APC / PE / REAL1316

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-130-351 (View supplier product page)
Size 200 μl
Price $530.00
Antigen APC
Clone REAL1316
Host cell line
Isotype IgG
Conjugate PE
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), IF, IHC
Description Clone REAL1316 is an antibody fragment derived from the full APC (DP2.5) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. | Clone REAL1316 recognizes the human APC (DP2.5) which plays a critical role in several cellular processes. The APC protein acts as a tumor suppressor. It promotes rapid degradation of CTNNB1 and participates in Wnt signaling pathway as a negative regulator. APC (DP2.5) is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers. | For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675). |
About APC and PE
APC This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Dec 2019]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
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PE Excitation and Emission Spectra