CD86 / FITC / PO3

Citations: (5)
Product Details
Supplier Bio-Rad
Catalog #: MCA2463F (View supplier product page)
Size 100 μg
Price $316.00
Antigen CD86
Clone PO3
Host Rat
Isotype IgG2b
Conjugate FITC
Target Species Mouse
Applications FC
Description RAT ANTI MOUSE CD86:FITC
About CD86 and FITC
CD86 This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Images
  CD86 FITC Bio-Rad antibody anti target:Mouse host:Rat isotype:IgG2b clone:PO3 Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD86:FITC (MCA2463F)

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD86:FITC (MCA2463F)
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FITC Excitation and Emission Spectra