CD8a / Pacific Blue / KT15

Citations: (24)
Product Details
Supplier Bio-Rad
Catalog #: MCA609PB (View supplier product page)
Size 100 Tests/1ml
Price $392.00
Antigen CD8a
Clone KT15
Host Rat
Isotype IgG2a
Conjugate Pacific Blue
Target Species Mouse
Applications FC
Description RAT ANTI MOUSE CD8 ALPHA:Pacific Blue®
About CD8a and Pacific Blue
CD8a The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]
Pacific Blue Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm.
Images
  CD8a Pacific Blue Bio-Rad antibody anti target:Mouse host:Rat isotype:IgG2a clone:KT15 <strong>Published customer image:</strong> <br>Rat anti Mouse CD4 antibody, clone KT15 used for t...

Published customer image:
Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide boost leads to loss of high avidity responses in vivo. A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFNγ elispot assay, D) normalization of responses to increasing peptide concentration in IFNγ elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments.

From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.

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Pacific Blue Excitation and Emission Spectra