CD169 / FITC / 3D6.112
CD169 / FITC / 3D6.112
Citations:
(46)
Product Details
| Supplier | Bio-Rad | |
|---|---|---|
| Catalog #: | MCA884F (View supplier product page) | |
| Size | 100 μg | |
| Price | $316.00 | |
| Antigen | CD169 | |
| Clone | 3D6.112 | |
| Host | Rat | |
| Isotype | IgG2a | |
| Conjugate | FITC | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | RAT ANTI MOUSE CD169:FITC |
About CD169 and FITC
| CD169 | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Images
|
Figure A. RPE conjugated Rat anti Mouse CD45R (MCA1258PE) and FITC conjugated Rat IgG2a isotype control (MCA1212F). Figure B. RPE conjugated Rat anti Mouse CD45R (MCA1258PE) and FITC conjugated Rat anti Mouse CD169 (MCA884F). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A). |
Citations
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FITC Excitation and Emission Spectra
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