CD40 / FITC / LOB7/6
CD40 / FITC / LOB7/6
Citations:
(47)
Product Details
| Supplier | Bio-Rad | |
|---|---|---|
| Catalog #: | MCA1590F (View supplier product page) | |
| Size | 100 μg | |
| Price | $316.00 | |
| Antigen | CD40 | |
| Clone | LOB7/6 | |
| Host | Mouse | |
| Isotype | IgG2a | |
| Conjugate | FITC | |
| Target Species | Human | |
| Applications | FC | |
| Description | MOUSE ANTI HUMAN CD40:FITC |
About CD40 and FITC
| CD40 | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Images
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Sandwich ELISA analysis of Human CD40 binding using Mouse anti Human CD40 antibody, clone 3B2 (MCA2788GA) as a capture reagent and biotinylated Mouse anti Human CD40 antibody, clone LOB7/6 (MCA1590B) as a detection reagent with purified Human CD40 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Undiluted serum and plasma samples are shown green and orange respectively. |
Citations
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Reviews & Ratings
FITC Excitation and Emission Spectra
$316.00
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