CD40 / Alexa Fluor 647 / LOB7/6
CD40 / Alexa Fluor 647 / LOB7/6
Product Details
| Supplier | Bio-Rad | |
|---|---|---|
| Catalog #: | MCA1590A647T (View supplier product page) | |
| Size | 25 Tests in 250 μl | |
| Price | $137.00 | |
| Antigen | CD40 | |
| Clone | LOB7/6 | |
| Host | Mouse | |
| Isotype | IgG2a | |
| Conjugate | Alexa Fluor 647 | |
| Target Species | Canine, Human | |
| Applications | FC | |
| Description | MOUSE ANTI HUMAN CD40:Alexa Fluor® 647 |
About CD40 and Alexa Fluor 647
| CD40 | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] | |
|---|---|---|
| Alexa Fluor 647 | Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye. |
Images
|
Sandwich ELISA analysis of Human CD40 binding using Mouse anti Human CD40 antibody, clone 3B2 (MCA2788GA) as a capture reagent and biotinylated Mouse anti Human CD40 antibody, clone LOB7/6 (MCA1590B) as a detection reagent with purified Human CD40 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Undiluted serum and plasma samples are shown green and orange respectively. |
Citations
| Additional Sources |
|
|---|
Reviews & Ratings
Alexa Fluor 647 Excitation and Emission Spectra
$137.00
Supplier Page
Supplier Page
