CD25 / Alexa Fluor 488 / PC61.5.3
CD25 / Alexa Fluor 488 / PC61.5.3
Citations:
(9)
Product Details
| Supplier | Bio-Rad | |
|---|---|---|
| Catalog #: | MCA1260A488 (View supplier product page) | |
| Size | 100 Tests in 1ml | |
| Price | $376.00 | |
| Antigen | CD25 | |
| Clone | PC61.5.3 | |
| Host | Rat | |
| Isotype | IgG1 | |
| Conjugate | Alexa Fluor 488 | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | RAT ANTI MOUSE CD25:Alexa Fluor® 488 |
About CD25 and Alexa Fluor 488
| CD25 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| Alexa Fluor 488 | Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A. |
Images
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Figure A. FITC conjugated rat anti mouse CD4 (MCA4635F) and RPE conjugated Rat IgG1 isotype control (MCA6004F). Figure B. FITC conjugated rat anti mouse CD4 (MCA4635F) and RPE conjugated rat anti mouse CD25 (MCA1260PE). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A). |
Citations
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Reviews & Ratings
Alexa Fluor 488 Excitation and Emission Spectra
$376.00
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