ABCA1 / FITC / 3A1-891.3

Citations: (1)
Product Details
Supplier Bio-Rad
Catalog #: MCA2878F (View supplier product page)
Size 100 μg
Price $282.00
Antigen ABCA1
Clone 3A1-891.3
Host Rat
Isotype IgG1
Conjugate FITC
Target Species Mouse
Applications FC
Description RAT ANTI MOUSE ABCA1:FITC
About ABCA1 and FITC
ABCA1 The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency. [provided by RefSeq, Sep 2019]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Images
  ABCA1 FITC Bio-Rad antibody anti target:Mouse host:Rat isotype:IgG1 clone:3A1-891.3 Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA (MCA2878)

Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA (MCA2878)
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FITC Excitation and Emission Spectra