CD25 / Alexa Fluor 647 / MEM-181
CD25 / Alexa Fluor 647 / MEM-181
Citations:
(13)
Product Details
| Supplier | Bio-Rad | |
|---|---|---|
| Catalog #: | MCA2127A647 (View supplier product page) | |
| Size | 100 Tests/1ml | |
| Price | $392.00 | |
| Antigen | CD25 | |
| Clone | MEM-181 | |
| Host | Mouse | |
| Isotype | IgG1 | |
| Conjugate | Alexa Fluor 647 | |
| Target Species | Human | |
| Applications | FC | |
| Description | MOUSE ANTI HUMAN CD25:Alexa Fluor® 647 |
About CD25 and Alexa Fluor 647
| CD25 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| Alexa Fluor 647 | Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye. |
Images
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Figure A. Alexa®647 conjugated mouse anti human CD4 (MCA1267A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa®647 conjugated mouse anti human CD4 (MCA1267A647) and RPE conjugated mouse anti human CD25 (MCA2127PE). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A) |
Citations
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Reviews & Ratings
Alexa Fluor 647 Excitation and Emission Spectra
$392.00
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