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V-type proton ATPase subunit F / Unconjugated / OTI1B8

Citations: (1)

Product Details

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP2-03498 (View supplier product page)
	Size	0.1 ml
	Price	$469.00
	Antigen	V-type proton ATPase subunit F
	Clone	OTI1B8
	Host	Mouse
	Isotype	IgG2a
	Conjugate	Unconjugated
	Target Species	Human
	Applications	FC, IHC-P, WB, ICC/IF, IHC

About V-type proton ATPase subunit F and Purified

	V-type proton ATPase subunit F	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq, Jul 2008]

Images

		Immunocytochemistry/Immunofluorescence: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] Staining of COS7 cells transiently transfected by pCMV6-ENTRY V-type proton ATPase subunit F.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human thyroid tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human liver tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
		Western Blot: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY V-type proton ATPase subunit F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-V-type proton ATPase subunit F.
		Flow Cytometry: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-V-type proton ATPase subunit F antibody, and then analyzed by flow cytometry.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lymph node tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human Kidney tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
		Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lung tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

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