Semaphorin 3E / Unconjugated / 400513

Product Details
Supplier R&D Systems
Catalog #: MAB32391 (View supplier product page)
Size 100 μg
Price $419.00
Antigen Semaphorin 3E
Clone 400513
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Target Species Human
Applications CyTOF, ICFC
Description Human Semaphorin 3E Antibody Intracellular Flow Cytometry = ICFC
About Semaphorin 3E and Purified
Semaphorin 3E Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
Images
  Semaphorin 3E Purified R&D Systems antibody anti target:Human host:Mouse isotype:IgG1 clone:400513 <html>Detection of Semaphorin 3E in Human T Cells by Flow Cytometry. Human T cells were treated f...

Detection of Semaphorin 3E in Human T Cells by Flow Cytometry. Human T cells were treated for 3-5 days with 1 µg/mL PHA then stained with Mouse Anti-Human Semaphorin 3E Monoclonal Antibody (Catalog # MAB32391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

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Purified Excitation and Emission Spectra