Antibody Search
OMIPs
eLearning 
Dye Directory
CD86 RealBlue 613 (BU63, Mouse) - Reactivity: Human
| Description |
The BU63 monoclonal antibody specifically recognizes CD86 which is also known as B70, B7-2, B7.2, LAB72 or CD28LG2. CD86 is a ~70-80 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD86 is expressed on monocytes, dendritic cells, Langerhans cells, activated B cells, and memory B cells. Like CD80 (B7-1), CD86 serves as a ligand for CD28 or CD152 (CTLA-4) expressed by T cells and can induce costimulatory or coinhibitory signals, respectively. The BU63 antibody can reportedly block the binding of other human CD86-specific antibodies including the 2331 (FUN-1) (complete block) or IT2.2 (partial block) antibodies. These results suggest that the BU63 antibody may bind to a similar or identical CD86 epitope as the 2331 (FUN-1) antibody but to a different, although spatially-related epitope compared with the IT2.2 antibody. |
|
|---|---|---|
| Conjugate | RealBlue 613 | |
| Clone | BU63 | |
| Target Species | Human | |
| Applications | FC | |
| Supplier | BD Biosciences | |
| Catalog # | Sign in to view product details, citations, and spectra | |
| Size | ||
| Price | ||
| Antigen | ||
| Host | ||
| Isotype |
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
RealBlue™ 613 (RB613) from BD Biosciences is a very bright alternative to PE-CF®594 and PE-Dazzle® 594 tandem fluorophores and has the advantage of discrete excitation off of the 488nm laser without much cross-beam excitation by the 532 or 561nm lasers. This limited cross-beam excitation will translate to lower complexity index scores and lower spillover spreading error in high parameter spectral flow cytometry applications. RB613 will peak in the same emission channel at 613nm as PE-CF®594 and PE-Dazzle® 594 tandem fluorophores.
| Additional Sources |
|
|---|
| Reviews |
|---|
