CD19 Monoclonal / NovaFluor Yellow 610 / HIB19

Product Details
Supplier Thermo Fisher Scientific
Catalog #: H004T02Y03-A (View supplier product page)
Size 25 Tests
Price $192.00
Antigen CD19
Clone HIB19
Host Mouse
Isotype IgG1 κ
Conjugate NovaFluor Yellow 610
Target Species Human
Applications FC
Description CD19 Monoclonal Antibody (HIB19), NovaFluor Yellow 610, eBioscience
About CD19 and NovaFluor Yellow 610
CD19 This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]
NovaFluor Yellow 610 The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliantâ„¢ family of fluorescent polymers from BD. NovaFluor Yellow 610 is designed to be discreetly excited by the 561 nm laser and has an excitation peak at 552 nm and an emission peak at 612 nm.
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NovaFluor Yellow 610 Excitation and Emission Spectra