Perforin / FITC / B-D48
Perforin / FITC / B-D48
Citations:
(2)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 353309 (View supplier product page) | |
| Size | 25T | |
| Price | $124.00 | |
| Antigen | Perforin | |
| Clone | B-D48 | |
| Host | Mouse | |
| Isotype | IgG1 | |
| Conjugate | FITC | |
| Target Species | Human | |
| Applications | ICS | |
| Description | FITC anti-human Perforin |
About Perforin and FITC
| Perforin | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Images
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Human peripheral blood lymphocytes were surface stained with CD8 APC, fixed with Fixation Buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer (10X), and then intracellularly stained with Perforin (clone B-D48) FITC (top), or mouse IgG1, κ FITC isotype control (bottom). |
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Citations
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FITC Excitation and Emission Spectra
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