CX3CL1 Monoclonal / APC / REA281

Citations: (3)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-108-080 (View supplier product page)
Size 100 Tests in 1 ml
Price $325.00
Antigen CX3CL1
Clone REA281
Host Human
Isotype IgG1
Conjugate APC
Target Species Human
Applications FC
Description Clone REA281 recognizes the human C-X3-C motif chemokine 1 (CX3CL1) antigen, a single-pass type I membrane protein, which is also known as fractalkine or neurotactin. CX3CL1 is expressed in a number of cell types including neurons, intestinal epithelium, and activated vascular endothelium and is structurally distinct from other chemokines. The membrane-anchored form of CX3CL1 induces tight, shear-resistant endothelial adhesion of leukocytes in a manner that is independent of integrin and Gi protein activation. Soluble forms of CX3CL1 generated by proteolytic cleavage with the disintegrin and metalloproteinases CD156c (ADAM10) and CD156b (ADAM17) were shown to promote conventional chemotaxis of T cells and monocytes, but not of neutrophils. The sole receptor for CX3CL1 is CX3CR1, which is widely expressed by leukocytes, including CD14+ cells of the monocyte-macrophage DC lineage and subsets of tissue-resident DCs and epidermal Langerhans cells. In addition to supporting leukocyte extravasation from the blood, the CX3CL1–CX3CR1 axis has been implicated in pathogenesis of inflammatory diseases such as atherosclerosis, rheumatoid arthritis, and renal fibrosis. CX3CL1 was also shown to promote extension of transepithelial dendrites by monocytes and/or DCs within intestinal lamina propria, for the purpose of antigen sensing in the intestinal lumen. | Additional information: Clone REA281 displays negligible binding to Fc receptors. |
About CX3CL1 and APC
CX3CL1 This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017]
APC Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.
Citations
PMID 9024663
PMID 10458770
PMID 24006262
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APC Excitation and Emission Spectra