FluoroFinder

	Supplier	BioLegend
	Catalog #:	506529 (View supplier product page)
	Size	25T
	Price	$159.00
	Antigen	IFN-gamma
	Clone	B27
	Host	Mouse
	Isotype	IgG1 κ
	Conjugate	PE-Dazzle 594
	Target Species	Human
	Applications	ICS
	Description	PE/Dazzle 594 anti-human IFN-gamma

	IFN-gamma	This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
	PE-Dazzle 594	PE-Dazzle® 594 (RPE-Dazzle 594) from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Dazzle 594. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Dazzle 594, which emitts light that can be captured with a 610/20 nm bandpass filter. PE-Dazzle 594 has an excitation peak at 565 nm and an emission peak at 610 nm, and is suitable alternative to PE-Texas Red, PE-eF610, PE-Alexa Fluor™ 610 or PE-CF®594.

		PMA+ionomycin-stimulated (in the presence of monensin, 6hrs) human peripheral blood lymphocytes were stained with CD3 APC, fixed, permeabilized, and then intracellularly stained with IFN-γ (clone B27) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom).