IgM Secondary Antibody / Alexa Fluor 647 / MHM-88
IgM Secondary Antibody / Alexa Fluor 647 / MHM-88
Citations:
(5)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 314535 (View supplier product page) | |
| Size | 25T | |
| Price | $165.00 | |
| Antigen | IgM | |
| Clone | MHM-88 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Alexa Fluor 647 | |
| Target Species | Human | |
| Applications | FC | |
| Description | Alexa Fluor 647 anti-human IgM |
About IgM and Alexa Fluor 647
| IgM | Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010] | |
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| Alexa Fluor 647 | Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye. |
Images
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Overnight cultured human peripheral blood mononuclear cells were stained with CD19 PE and IgM (clone MHM-88) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom). Data shown was gated on lymphocyte population. |
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Citations
| PMID 24778443 | ||
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| Additional Sources |
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Reviews & Ratings
Alexa Fluor 647 Excitation and Emission Spectra
$165.00
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