FluoroFinder

	LAG-3	Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
	Alexa Fluor 647	Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

		CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells (PBMCs) were stained with CD8 FITC and CD223 (clone 11C3C65) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).