CD20 / Brilliant Violet 785 / 2H7

Citations: (4)
Product Details
Supplier BioLegend
Catalog #: 302355 (View supplier product page)
Size 25T
Price $215.00
Antigen CD20
Clone 2H7
Host Mouse
Isotype IgG2b κ
Conjugate Brilliant Violet 785
Target Species Cynomolgus macaque, Human, Rhesus macaque
Applications FC
Description Brilliant Violet 785 anti-human CD20
About CD20 and Brilliant Violet 785
CD20 This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Brilliant Violet 785 Brilliant™ Violet 785 (BV785) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV785 has an excitation peak at 405 nm and an emission peak at 785 nm. BV785 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV785 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD20 Brilliant Violet 785 BioLegend antibody anti target:Cynomolgus macaque, Human, Rhesus macaque host:Mouse isotype:IgG2b κ clone:2H7

Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 785™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 785™ isotype control (open histogram).

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Brilliant Violet 785 Excitation and Emission Spectra