CD115 / PerCP-Cy5.5 / AFS98
CD115 / PerCP-Cy5.5 / AFS98
Citations:
(10)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 135526 (View supplier product page) | |
| Size | 100 μg | |
| Price | $335.00 | |
| Antigen | CD115 | |
| Clone | AFS98 | |
| Host | Rat | |
| Isotype | IgG2a κ | |
| Conjugate | PerCP-Cy5.5 | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | PerCP/Cyanine5.5 anti-mouse CD115 (CSF-1R) |
About CD115 and PerCP-Cy5.5
| CD115 | The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma. [provided by RefSeq, Mar 2017] | |
|---|---|---|
| PerCP-Cy5.5 | PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710. |
Images
|
Thioglycolate-elicited BALB/c peritoneal macrophages were stained with CD11b FITC and CD115 (CSF-1R, clone AFS98) PerCP/Cyanine5.5 (left), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right). |
Citations
| PMID 22393124 | ||
|---|---|---|
| Additional Sources |
|
Reviews & Ratings
PerCP-Cy5.5 Excitation and Emission Spectra
$335.00
Supplier Page
Supplier Page
