IFN-gamma / PerCP-Cy5.5 / B27

Citations: (4)
Product Details
Supplier BioLegend
Catalog #: 506527 (View supplier product page)
Size 25T
Price $171.00
Antigen IFN-gamma
Clone B27
Host Mouse
Isotype IgG1 κ
Conjugate PerCP-Cy5.5
Target Species Human
Applications ICS
Description PerCP/Cyanine5.5 anti-human IFN-gamma
About IFN-gamma and PerCP-Cy5.5
IFN-gamma This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
PerCP-Cy5.5 PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
Images
  IFN-gamma PerCP-Cy5.5 BioLegend antibody anti target:Human host:Mouse isotype:IgG1 κ clone:B27

PMA+ionomycin-stimulated human peripheral blood lymphocytes (6-hours), in the presence of monensin, were stained with CD3 APC, fixed, permeabilized, and then intracellularly stained with IFN-γ (clone B27) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
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PerCP-Cy5.5 Excitation and Emission Spectra