Perforin / Brilliant Violet 421 / dG9
Perforin / Brilliant Violet 421 / dG9
Citations:
(16)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 308122 (View supplier product page) | |
| Size | 100T | |
| Price | $424.00 | |
| Antigen | Perforin | |
| Clone | dG9 | |
| Host | Mouse | |
| Isotype | IgG2b κ | |
| Conjugate | Brilliant Violet 421 | |
| Target Species | Human | |
| Applications | ICS | |
| Description | Brilliant Violet 421 anti-human Perforin |
About Perforin and Brilliant Violet 421
| Perforin | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] | |
|---|---|---|
| Brilliant Violet 421 | Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Human peripheral blood lymphocytes were surface stained with CD8 APC, fixed with Fixation Buffer, permeabilized with Permeabilization Wash Buffer, and then intracellularly stained with perforin (clone dG9) Brilliant Violet 421™ (top), or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom). |
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Citations
| PMID 19704116 | ||
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| Additional Sources |
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Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra
$424.00
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