FluoroFinder

	Supplier	BioLegend
	Catalog #:	145807 (View supplier product page)
	Size	25 μg
	Price	$176.00
	Antigen	PLZF
	Clone	9E12
	Host	Armenian Hamster
	Isotype	IgG
	Conjugate	PerCP-Cy5.5
	Target Species	Mouse
	Applications	ICS
	Description	PerCP/Cyanine5.5 anti-mouse PLZF

	PLZF	This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
	PerCP-Cy5.5	PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.

		BALB/c thymocytes were surface stained with CD3 FITC, then fixed and permeabilized using Fixation buffer and Permeabilization wash buffer, and then were stained with PLZF ( clone 9E12) PerCP/Cy5.5 (top) or Armenian hamster IgG PerCP/Cy5.5 Isotype control (bottom)