FluoroFinder

	CD20	This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
	Alexa Fluor 647	Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

		C57BL/6 mouse splenocytes were stained with IgD FITC and CD20 (clone SA275A11) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa FluorÂ® 647 isotype control (bottom).

		C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat Serum for one hour at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD20 (clone SA275A11) Alexa Fluor® 647 (red), 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) and 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 594 (blue) at 4°C overnight. The image was captured with a 10X objective.