CD5 / Brilliant Violet 605 / L17F12

Citations: (3)
Product Details
Supplier BioLegend
Catalog #: 364020 (View supplier product page)
Size 100T
Price $418.00
Antigen CD5
Clone L17F12
Host Mouse
Isotype IgG2a κ
Conjugate Brilliant Violet 605
Target Species Human
Applications FC
Description Brilliant Violet 605 anti-human CD5
About CD5 and Brilliant Violet 605
CD5 This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
Brilliant Violet 605 Brilliant™ Violet 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. BV605 is most commonly used in flow cytometry and is spectrally similar to SuperBright 600 (Thermo Fisher), SuperNova v605 (Beckman Coulter) and StarBright Violet 610 (Bio-Rad). BV605 is considered to be relatively bright and should be used on antigens of moderate to low abudance. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD5 Brilliant Violet 605 BioLegend antibody anti target:Human host:Mouse isotype:IgG2a κ clone:L17F12

Human peripheral blood lymphocytes were stained with CD5 (clone L17F12) Brilliant Violet 605™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).

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Brilliant Violet 605 Excitation and Emission Spectra