CD56 / Brilliant Violet 650 / HCD56
CD56 / Brilliant Violet 650 / HCD56
Citations:
(6)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 318344 (View supplier product page) | |
| Size | 100T | |
| Price | $424.00 | |
| Antigen | CD56 | |
| Clone | HCD56 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Brilliant Violet 650 | |
| Target Species | Human | |
| Applications | FC | |
| Description | Brilliant Violet 650 anti-human CD56 (NCAM) |
About CD56 and Brilliant Violet 650
| CD56 | This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2020] | |
|---|---|---|
| Brilliant Violet 650 | Brilliant™ Violet 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique Stokes shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot® 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 650™ (top) or mouse IgG1, κ Brilliant Violet 650™ isotype control (bottom). |
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Citations
| PMID 21633088 | ||
|---|---|---|
| PMID 25687281 | ||
| PMID 25687283 | ||
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 650 Excitation and Emission Spectra
$424.00
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