Helios / PE-Cy7 / 22F6

Citations: (3)
Product Details
Supplier BioLegend
Catalog #: 137235 (View supplier product page)
Size 25T
Price $194.00
Antigen Helios
Clone 22F6
Host Armenian Hamster
Isotype IgG
Conjugate PE-Cy7
Target Species Human, Mouse
Applications ICS
Description PE/Cyanine7 anti-mouse/human Helios
About Helios and PE-Cy7
Helios This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
PE-Cy7 PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy7, which emits light that can be captured with a 780/60 nm bandpass filter. PE-CY7 has an excitation peak at 565 nm and an emission peak at 778 nm, and is a suitable alternative to PE-Vio®770 (Miltenyi) and cFluor BYG781 (Cytek).
Images
  Helios PE-Cy7 BioLegend antibody anti target:Human, Mouse host:Armenian Hamster isotype:IgG clone:22F6

C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Helios (clone 22F6) PE/Cy7.
Citations
PMID 20181882
PMID 22991461
PMID 23063892
PMID 24498287
PMID 24820384
PMID 24852566
PMID 25108241
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PE-Cy7 Excitation and Emission Spectra