FluoroFinder

	Supplier	BioLegend
	Catalog #:	108139 (View supplier product page)
	Size	50 μg
	Price	$273.00
	Antigen	Ly-6A/Ly-6E
	Clone	D7
	Host	Rat
	Isotype	IgG2a κ
	Conjugate	Brilliant Violet 785
	Target Species	Mouse
	Applications	FC
	Description	Brilliant Violet 785 anti-mouse Ly-6A/E (Sca-1)

	Ly-6A/Ly-6E	This gene belongs to the human Ly6 gene family and encodes a glycosylphosphatidyl-inositol (GPI)-anchored cell surface protein. The protein plays an important role in T cell physiology, oncogenesis and immunological regulation. The protein is also involved in modulation of viral infection by coronaviruses, SARS-CoV, MERS-CoV and SARS-CoV-2. [provided by RefSeq, Aug 2021]
	Brilliant Violet 785	Brilliant™ Violet 785 (BV785) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV785 has an excitation peak at 405 nm and an emission peak at 785 nm. BV785 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV785 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.

		C57BL/6 mouse splenocytes were stained with Ly-6A/E (clone D7) Brilliant Violet 785™ (filled histogram) or rat IgG2a, κ Brilliant Violet 785™ isotype control (open histogram).
		C57BL/6 mouse bone marrow cells were stained with Ly-6A/E (clone D7) Brilliant Violet 785™ (filled histogram) or rat IgG2a, κ Brilliant Violet 785™ isotype control (open histogram). Data shown was gated on the lymphoid cell population.