PDCD4 / FITC / 4C2

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP3-18224F (View supplier product page)
Size 100 μl
Price $449.00
Antigen PDCD4
Clone 4C2
Host Mouse
Isotype IgG1 κ
Conjugate FITC
Target Species Human
Applications WB, FC, ELISA, ICC, IF
Description PDCD4 Antibody (4C2) [FITC]
About PDCD4 and FITC
PDCD4 This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra