ErbB2 / Alexa Fluor 647 / ERBB2/7158R

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP3-24037AF647 (View supplier product page)
Size 100 μl
Price $599.00
Antigen ErbB2
Clone ERBB2/7158R
Host Rabbit
Isotype IgG κ
Conjugate Alexa Fluor 647
Target Species Human
Applications IHC-P
Description ErbB2/Her2 Antibody (ERBB2/7158R) [Alexa Fluor® 647]
About ErbB2 and Alexa Fluor 647
ErbB2 This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
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Alexa Fluor 647 Excitation and Emission Spectra