FluoroFinder

	Supplier	BioLegend
	Catalog #:	648306 (View supplier product page)
	Size	100T
	Price	$405.00
	Antigen	Bcl-6
	Clone	IG191E/A8
	Host	Mouse
	Isotype	IgG1
	Conjugate	Alexa Fluor 647
	Target Species	Human, Mouse
	Applications	ICS
	Description	Alexa Fluor 647 anti-mouse/human Bcl-6

	Bcl-6	The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
	Alexa Fluor 647	Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

		C57BL/6 lymph node cells were immunized with CFA for 12 days, fixed, and permeabilized with BioLegend Nuclear Factor Fixation and Permeabilization Buffer Set, then stained with CD19 FITC, IgD PerCP/Cy5.5, and Bcl-6 (clone IG191E/A8) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom). Dot plots are gated on CD19⁺ lymphocytes.