IgG Secondary Antibody / DyLight 650 / Polyclonal

Product Details
Supplier Thermo Fisher Scientific
Catalog #: SA510029 (View supplier product page)
Size 500 μg
Price $149.00
Antigen IgG
Clone Polyclonal
Host Donkey
Isotype IgG
Conjugate DyLight 650
Target Species Rat
Applications FC, ICC, IF, IP, WB
Description Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate. Thermo Scientific Pierce Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.FACS 1:25 - 1:100, ICC 1:50 - 1:2,000, IF 1:50 - 1:2,000, IHC 1:50 - 1:2,000, IP Assay Dependent, WB 1:5,000 - 1:20,000
About IgG and DyLight 650
IgG This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
DyLight 650 DyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor™ 647 and Cy5. DyLight™ 650 is most commonly used in flow cytometery and fluorescence microscopy applications.
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DyLight 650 Excitation and Emission Spectra