53BP1 / DyLight 650 / Polyclonal

Product Details
Supplier Thermo Fisher Scientific
Catalog #: PA522791 (View supplier product page)
Size 100 μl
Price $354.00
Antigen 53BP1
Clone Polyclonal
Host Rabbit
Isotype IgG
Conjugate DyLight 650
Target Species Human, Mouse
Applications ICC, WB, IF
Description 53BP1 Polyclonal Antibody, DyLight 650 conjugate. P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.ICC 1:100-1:1000, WB 1:1000-1:10,000, IF 1:100-1:1000
About 53BP1 and DyLight 650
53BP1 This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
DyLight 650 DyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor™ 647 and Cy5. DyLight™ 650 is most commonly used in flow cytometery and fluorescence microscopy applications.
Citations
Additional
Sources
Reviews & Ratings
Submit a Review

DyLight 650 Excitation and Emission Spectra