beta Galactosidase / FITC / Polyclonal

Product Details
Supplier Thermo Fisher Scientific
Catalog #: PA126791 (View supplier product page)
Size 1 mg
Price $360.00
Antigen beta Galactosidase
Clone Polyclonal
Host Rabbit
Isotype IgG
Conjugate FITC
Target Species All Species
Applications ELISA, WB, Immunoassay
Description beta Galactosidase Polyclonal Antibody, FITC conjugate. This highly active polyclonal antibody is directed against the bacterial form of the enzyme and may be used to detect any beta-galactosidase expression by the lacZ gene. This reagent is specifically designed for screening and recovery of fusion porteins from expression systems with lacZ gene cloning sites.IHC (F) 1:200-1:2000, IM Assay Dependent, ELISA Assay Dependent, WB Assay-dependent
About beta Galactosidase and FITC
beta Galactosidase This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra