Carbonic Anhydrase II / FITC / 4

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-89523F (View supplier product page)
Size 100 μl
Price $549.00
Antigen Carbonic Anhydrase II
Clone 4
Host Rabbit
Isotype IgG
Conjugate FITC
Target Species Human
Applications ELISA
Description Carbonic Anhydrase II/CA2 Antibody (004) [FITC]
About Carbonic Anhydrase II and FITC
Carbonic Anhydrase II The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra