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CD86 RealBlue 744 (2331 (FUN-1), Mouse) - Reactivity: Baboon, Cynomolgus macaque, Human, Rhesus macaque
Product Details
| Description |
The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells. Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (Anti-CD86) antibodies specifically recognize the same molecule, they react with different epitopes. CD86 is a ligand for CD28 and CTLA-4 and plays an important role in costimulation of T cells in primary immune response. The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies. |
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|---|---|---|
| Conjugate | RealBlue 744 | |
| Clone | 2331 (FUN-1) | |
| Target Species | Baboon, Cynomolgus macaque, Human, Rhesus macaque | |
| Applications | FC | |
| Supplier | BD Biosciences | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About CD86
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
About RealBlue 744
RealBlue™ 744 from BD Biosciences is a novel multimer fluorophore for use in spectral flow cytometry applications with an optimal excitation peak at 488nm and an emission peak at 744nm. The emission peak fits neatly between the PerCP-Cy5.5/ StarBright Blue 700/ RealBlue™ 705 and the RealBlue™ 780 emission peaks which means it does not fall into a standard filter set on conventional, non-spectral flow cytometers. It exhibits minimal cross-beam excitation off the 355nm UV, 405nm violet, 561nm yellow/green and 633/647nm red lasers resulting in low potential for spillover spreading error in a multicolor flow cytometry assay.
RealBlue™ 744 from BD Biosciences is a novel multimer fluorophore for use in spectral flow cytometry applications with an optimal excitation peak at 488nm and an emission peak at 744nm. The emission peak fits neatly between the PerCP-Cy5.5/ StarBright Blue 700/ RealBlue™ 705 and the RealBlue™ 780 emission peaks which means it does not fall into a standard filter set on conventional, non-spectral flow cytometers. It exhibits minimal cross-beam excitation off the 355nm UV, 405nm violet, 561nm yellow/green and 633/647nm red lasers resulting in low potential for spillover spreading error in a multicolor flow cytometry assay.
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