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CD86 RealBlue 744 (BU63, Mouse) - Reactivity: Human
| Description |
The BU63 monoclonal antibody specifically recognizes CD86 which is also known as B70, B7-2, B7.2, LAB72 or CD28LG2. CD86 is a ~70-80 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD86 is expressed on monocytes, dendritic cells, Langerhans cells, activated B cells, and memory B cells. Like CD80 (B7-1), CD86 serves as a ligand for CD28 or CD152 (CTLA-4) expressed by T cells and can induce costimulatory or coinhibitory signals, respectively. The BU63 antibody can reportedly block the binding of other human CD86-specific antibodies including the 2331 (FUN-1) (complete block) or IT2.2 (partial block) antibodies. These results suggest that the BU63 antibody may bind to a similar or identical CD86 epitope as the 2331 (FUN-1) antibody but to a different, although spatially-related epitope compared with the IT2.2 antibody. |
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|---|---|---|
| Conjugate | RealBlue 744 | |
| Clone | BU63 | |
| Target Species | Human | |
| Applications | FC | |
| Supplier | BD Biosciences | |
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| Isotype |
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
RealBlue™ 744 from BD Biosciences is a novel multimer fluorophore for use in spectral flow cytometry applications with an optimal excitation peak at 488nm and an emission peak at 744nm. The emission peak fits neatly between the PerCP-Cy5.5/ StarBright Blue 700/ RealBlue™ 705 and the RealBlue™ 780 emission peaks which means it does not fall into a standard filter set on conventional, non-spectral flow cytometers. It exhibits minimal cross-beam excitation off the 355nm UV, 405nm violet, 561nm yellow/green and 633/647nm red lasers resulting in low potential for spillover spreading error in a multicolor flow cytometry assay.
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