IgG Secondary Antibody / Alexa Fluor 546 / Polyclonal

Product Details
Supplier Thermo Fisher Scientific
Catalog #: A21089 (View supplier product page)
Size 500 μl
Price $159.00
Antigen IgG
Clone Polyclonal
Host Goat
Isotype IgG
Conjugate Alexa Fluor 546
Target Species Human
Applications IF, FC
Description Goat anti-Human IgG (H+L) Secondary Antibody, Alexa Fluor® 546 conjugate. Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.IF 1-10 µg/mL, FACS 1-10 µg/mL
About IgG and Alexa Fluor 546
IgG This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
Alexa Fluor 546 Alexa Fluor™ 546 (AF546, Alexa 546) has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor® 555 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 546 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
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Alexa Fluor 546 Excitation and Emission Spectra