NOV / Biotin / Polyclonal

Product Details
Supplier PeproTech
Catalog #: 500-P257Bt (View supplier product page)
Size 25 μg - 1 mg
Price $195.00
Antigen NOV
Clone Polyclonal
Host Rabbit
Isotype IgG
Conjugate Biotin
Target Species Human
Applications WB, ELISA, Capture
Description To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. To detect hNOV by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech's Polyclonal Anti-Human NOV (500-P257) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNOV. To detect hNOV by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNOV.Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNOV. Anti-Human NOV specific antibody was purified by affinity chromatography and then biotinylated.
About NOV and Biotin
NOV The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]
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Biotin Excitation and Emission Spectra