sCD40 Ligand / Biotin / Polyclonal

Product Details
Supplier PeproTech
Catalog #: 500-P142GBt (View supplier product page)
Size 25 μg - 1 mg
Price $195.00
Antigen sCD40 Ligand
Clone Polyclonal
Host Goat
Isotype IgG
Conjugate Biotin
Target Species Human
Applications WB, ELISA, Capture
Description To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. To detect hsCD40L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech's Polyclonal Anti-Human sCD40L (500-P142G) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsCD40L. To detect hsCD40L by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsCD40L.Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L. Anti-Human sCD40L specific antibody was purified by affinity chromatography and then biotinylated.
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Biotin Excitation and Emission Spectra