CX3CL1 / PE / 51637
CX3CL1 / PE / 51637
Citations:
(6)
Product Details
| Supplier | R&D Systems | |
|---|---|---|
| Catalog #: | IC365P (View supplier product page) | |
| Size | 100 Tests | |
| Price | $369.00 | |
| Antigen | CX3CL1 | |
| Clone | 51637 | |
| Host | Mouse | |
| Isotype | IgG1 | |
| Conjugate | PE | |
| Target Species | Human | |
| Applications | FC, ICFC | |
| Description | Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody Intracellular Flow Cytometry = ICFC |
About CX3CL1 and PE
| CX3CL1 | This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017] | |
|---|---|---|
| PE | Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts. |
Images
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Detection of CX3CL1/Fractalkine in NS0 Cell Line Transfected with His-tagged Human CX3CL1 by Flow Cytometry. NS0 cell line transfected with His-tagged human CX3CL1 was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A) and either (A) Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Monoclonal Antibody (Catalog # IC365P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Citations
| PMID 14612547 | ||
|---|---|---|
| PMID 17548644 | ||
| PMID 18725411 | ||
| PMID 18799722 | ||
| PMID 25587706 | ||
| PMID 26276874 | ||
| Additional Sources |
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Reviews & Ratings
PE Excitation and Emission Spectra
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