Granzyme B Monoclonal / PerCP / 351927
Granzyme B Monoclonal / PerCP / 351927
Product Details
| Supplier | R&D Systems | |
|---|---|---|
| Catalog #: | IC2906C (View supplier product page) | |
| Size | 100 Tests | |
| Price | $429.00 | |
| Antigen | Granzyme B | |
| Clone | 351927 | |
| Host | Mouse | |
| Isotype | IgG2a | |
| Conjugate | PerCP | |
| Target Species | Human, Mouse | |
| Applications | FC, ICFC | |
| Description | Human Granzyme B PerCP-conjugated Antibody Intracellular Flow Cytometry = ICFC |
About Granzyme B and PerCP
| Granzyme B | This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016] | |
|---|---|---|
| PerCP | Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter. PerCP exhibits a large Stokes' Shift, with an excitation peak at 482 nm and an emission peak at 675 nm. PerCP is was historically used in flow cytometry, however it is highly susceptible to photobleaching and has poor stability. Alternatives like BB700, NovaFluor Blue 690 or PerCP-eFluor™ 710 are preferred. PerCP is a generic dye that has no sole manufacturer. |
Images
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Detection of Granzyme B in NK-92 Human Cell Line by Flow Cytometry. NK-92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PerCP-conjugated Monoclonal Antibody (Catalog # IC2906C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Citations
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Reviews & Ratings
PerCP Excitation and Emission Spectra
$429.00
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