Brachyury / PE /
Brachyury / PE /
Citations:
(2)
Product Details
| Supplier | R&D Systems | |
|---|---|---|
| Catalog #: | IC2085P (View supplier product page) | |
| Size | 100 Tests | |
| Price | $409.00 | |
| Antigen | Brachyury | |
| Clone | ||
| Host | Goat | |
| Isotype | IgG | |
| Conjugate | PE | |
| Target Species | Human, Mouse, Porcine | |
| Applications | FC, WB, ICFC, ICC | |
| Description | Human/Mouse Brachyury PE-conjugated Antibody Intracellular Flow Cytometry = ICFC |
About Brachyury and PE
| Brachyury | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018] | |
|---|---|---|
| PE | Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts. |
Images
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Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Citations
| PMID 19265664 | ||
|---|---|---|
| PMID 27245508 | ||
| PMID 29781215 | ||
| Additional Sources |
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Reviews & Ratings
PE Excitation and Emission Spectra
$409.00
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