Brachyury / Alexa Fluor 488 /
Brachyury / Alexa Fluor 488 /
Citations:
(10)
Product Details
| Supplier | R&D Systems | |
|---|---|---|
| Catalog #: | IC2085G (View supplier product page) | |
| Size | 100 Tests | |
| Price | $439.00 | |
| Antigen | Brachyury | |
| Clone | ||
| Host | Goat | |
| Isotype | IgG | |
| Conjugate | Alexa Fluor 488 | |
| Target Species | Human, Mouse | |
| Applications | FC, ICFC | |
| Description | Human/Mouse Brachyury Alexa Fluor® 488-conjugated Antibody Intracellular Flow Cytometry = ICFC |
About Brachyury and Alexa Fluor 488
| Brachyury | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018] | |
|---|---|---|
| Alexa Fluor 488 | Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A. |
Images
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Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Citations
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Alexa Fluor 488 Excitation and Emission Spectra
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