CD19 StarBright Yellow 720 (6D5, Rat) - Reactivity: Mouse

About CD19
This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]

About StarBright Yellow 720
StarBright Yellow 720 (SBY720) Dye is a bright 561 nm excitable dye with a unique and narrow emission. It can be used in place of PE-Cy5.5 and PE-A700 in multicolor panels as it is brighter, has reduced 488 nm excitation, and is fixable in PFA and alcohol-based buffers without loss of signal or changes to the spectra. Furthermore, as it is not a traditional tandem dye it is not prone to changes in emission over time due to acceptor and donor uncoupling, giving you reproducible data. Like all StarBright Dyes, SBY720 can be multiplexed with other StarBright Dyes or other fluorophores without the need for a special buffer and can be premixed in a panel and stored at 4⁰C for over a month without changes to staining.