CD19 StarBright Yellow 605 (6D5, Rat) - Reactivity: Mouse

About CD19
This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]

About StarBright Yellow 605
StarBright Yellow 605 (SBY605) Dye is a bright 561 nm excitable dye with a unique and narrow emission that unlike PE-tandems with similar emissions, is not excited by the 488 nm laser. Suitable for use in conventional and full spectrum flow cytometry and compatible with other StarBright Dyes without the requirement for a special buffer, it is as bright as PE-Dazzle594 when excited by the 561 nm laser. SBY605 is not a traditional tandem dye so unlike PE-based tandems can be fixed in PFA or alcohol-based fixatives with no loss of signal or changes to the spectral profile giving you reduced spillover and more flexibility in your experiments.