L1CAM / APC / REAL1365

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-132-024 (View supplier product page)
Size 200 μl
Price $530.00
Antigen L1CAM
Clone REAL1365
Host cell line
Isotype IgG
Conjugate APC
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), IHC, IF
Description Clone REAL1365 is an antibody fragment derived from the full CD171 (L1CAM) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. | Clone REAL1365 recognizes human CD171 (L1CAM), a single pass type I membrane glycoprotein belonging to the immunoglobulin superfamily. Also known as L1CAM (L1 cell adhesion molecule) or NCAM-L1, CD171 belongs to a subgroup of cell adhesion molecules, which consist of six Ig-like domains, five fibronectin type III (FNIII)-like domains, a transmembrane region, and a cytoplasmic domain. Expression of CD171 is found on tetanus-toxin positive neurons, endothelial cells, certain epithelial cells, reticular fibroblasts, and several malignant tumors including colon and breast carcinomas, colon melanoma, tumor cells of neuronal, and mesothelial origin, where a role of CD171 in augmenting tumor growth has been demonstrated. Thus, CD171 is considered a novel marker for carcinoma progression. CD171 displays binding to a number of ligands including homophilic interaction with itself and heterophilic binding to integrins, other cell adhesion molecules, extracellular matrix molecules. It is involved in the development of the nervous system and regulates processes such as neuron–neuron adhesion, myelination, axonal guidance, and neuronal migration. | For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675). |
About L1CAM and APC
L1CAM The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]
APC Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.
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APC Excitation and Emission Spectra